The Punnett Squares and the different crosses seemed to be a point of difficulty in class today, so here is a recap and some more tips regarding the process of it all!
Brief History of Mendel: Mendel was a monk who discovered the idea of traits and genes and the mechanism by which they are transferred through the generations. Using pea plants (lots and lots… and LOTS of pea plants), and hand-counting all of his results, he studied the theories of dominance, as well as establishing the laws of segregation and independent assortment. Several factors about Mendel’s experiment made his evidence and conclusions convincing:
First, he was using Visible Phenotypic Traits, and fortunately for Mendel, the seven traits he picked to study behaved according to his theories of dominance.
Second, he was using a species of plants that Provided Plenty of Viable Offspring Each Generation, so his data wasn’t based on several small numbers and possible fluke cases.
And Thirdly, he was sure to isolate male plants from female plants and Controlled Plant Crosses so the data he received was under his control and accidental self-fertilizations could be avoided.
Though he experimented with all seven traits, we focused on his experiments dealing with wrinkled and smooth pea coats. Mendel began his experiment with a cross of a true-breeding smooth parent and a true-breeding wrinkled parent. The progeny, or F1 Generation, yielded only smooth plants. Thus, Mendel could deny the idea of blending (at this moment in the history of genetics at least). And, when he self-crossed the F1 generation, he saw a phenotypic ratio of 3/4 smooth peas to 1/4 wrinkled peas. Genotypically, this generation consisted of 1/4 Homozygous Dominant, 1/2 Heterozygous, and 1/4 Homozygous Recessive progeny.
Mendel concluded that the information for the texture of the pea coat was present in two forms, or what we now call Alleles. These alleles behaved according to the laws of segregation and independent assortment, which was supported by the F2 generation, and abided by the theory of dominance.
Some tips for your homework!
If you’re having trouble identifying the genotypes of the parents, look for answers in the progeny. Try to identify what phenotype is recessive and which is dominant, and perform your own hypothetical test crosses. Compare your results with what might be the parents’ genotypes, and try to find a logical match!
Remember that each pair of alleles separates at gametogenesis. Therefore, when you are doing a cross, remember to separate the alleles for both parents!
Also be sure to look at the fractions of genotype or phenotype ratios in your progeny! If they are not divided into fourths… perhaps there is more than one trait you’re dealing with!